The Biobrio 1(1), 2014

EFFECT OF DIFFERENT PHYTOHORMONES IN CULTURE OF EXPLANTS OF SPONDIAS MANGIFERA (L.)

INDU KUMARI AND R. K. PANDEY

ABSTRACT:

Spondias mangifera L. (Family: Anacardiaceae) commonly known as Amra is an economically important medicinal plant and found all over India. Explants of S. mangifera L. were cultured on MS basal media to obtain callus and plantlets. MS media supplemented with different growth regulators like 2, 4-D, IBA, IAA, NAA, BAP, Kinten Zeatin either alone or in combination. Cultures were kept on 25+ 5° C and 12 hrs photoperiod. White callus was observed on different concentration of phytohormones. Only callusing and no organogenesis was seen on medium containing 2, 4-D. The best -1 result was observed at 2.5 mgL concentration of auxins. Cytokinins also induce callus formation and shoot differentiation. BAP was found to be the most effective cytokinin for shoot differentiation in combination with auxin. Most suitable medium noted for shoot formation from explants (shoot tips or leaves) was MS media supplemented with BAP (2 -1 -1 mgL ) and IAA (1 mgL ). Other effective combination for shoot differentiation was with -1 -1 -1 -1 BAP (3 mgL ) and IAA (2 mgL ) and other was MS with BAP (1 mgL ) and NAA (0.5 mgL ). -1 Shoot developed was rooted the best in MS media with 0.5 mgL NAA.

Keywords:    

Spondias mangifera L., MS medium, Explant, Auxin, Cytokinin, Callus

Full Text